The development of a reusable microfluidic device for sorting and manipulating cells and other micro/nano meter scale objects will make biomedical diagnosis of diseases cheaper and more convenient in regions where medical facilities are sparse or cost is prohibitive. Researchers at Penn State have recently filed a patent to develop such a device.
Based on gentle acoustic vibrations, the device, called acoustic tweezers, is the work of Penn State professor of engineering science and mechanics, Tony Huang and his students. Their work is the cover article for the current issue of the journal Lab on a Chip.
“We believe our acoustic tweezers have tremendous potential, especially in diagnostics, with some applications also in therapeutics,” Huang said. “Our current device works well, but to be used in diagnostics, the whole device has to be disposed of after one use. We have now found a way to separate the fluid-containing part of the device from the much more expensive ultrasound-producing piezoelectric substrate. This makes disposable acoustic tweezers possible.”
Huang believes that the disposable plastic portion of the device can be manufactured for as little as 25 cents per unit. Even with the addition of electronics for diagnosis, which could be easily added off the shelf, he foresees a manufacturing cost of a few dollars for the complete permanent system, which could then be used over and over with the simple replacement of the plastic microfluidic channels.
“We want to push this toward a commercial product, something that can help people and benefit society,” Huang said.
In the prior device, the microfluidic channel was permanently bound to the substrate and the ultrasound would be radiated directly into the fluid. In the new device, there is an intervening layer, but the ultrasound force is strong enough to manipulate the cells and to pattern them. This patterning of cells is especially important in studying cell-to-cell communication in biology labs or for drug screening.
“In drug screening, you want to examine how cells respond to drugs. With our acoustic tweezers, we can create a high throughput of single cells and see how they respond to drugs. Or, we create all types of cell assemblies and see how they respond to drugs, much more like how it would work inside the body,” said Feng Guo, a Ph.D. student in Huang’s group and lead author of the paper “Reusable acoustic tweezers for disposable devices.”
In addition to its use in diagnosing diseases such as HIV and tuberculosis, both of which are endemic in resource-poor regions of the world, the device should find widespread use in hospitals, clinics, biology labs and the home due to its low cost and ease of use.
Additional authors are Yuliang Xie, Sixing Li, James Lata, Liqiang Ren, Zhangming Mao, Baiyang Ren, Mengxi Wu and Adem Ozcelik, all of Penn State..
Funding was provided by the National Institutes of Health, the National Science Foundation, and the Penn State Center for Nanoscale Science(MRSEC). Components of the work were performed in the Penn State Materials Research Institute’s Nanofabrication Laboratory.