There’s a short window for detecting influenza virus, because as the infection takes hold – the concentration of the virus lessens. So if the patient isn’t tested soon after exposure, conventional methods run the risk of a giving a false negative result.
A new method under development at KTH Royal Institute of Technologyhowever could provide sensitive detection of the virus, much faster than the nasal swabbing tests used today.
All it takes is for patients to breathe into a bottle.
The EU-funded project is two-fold, and the results were published recently in the scientific journals,and . Led by KTH , a research team from KTH – in collaboration with the University of Antwerp, the University of Leuven, and Janssen Diagnostics – from patients’ breath, in vitroand in pre-clinical studies. In a parallel effort, the researchers have developed a .
van der Wijngaart describes the “femtolitre well array biosensor” as a configuration of tens of thousands of wells, each smaller than the size of a single red blood cell, that capture the proteins. This part of the EU-funded project has proven successful in singling out non-virus molecules, but van der Wijngaart says development will continue for some time. The researchers involved include Gaspard Pardon (a former post doc in Micro and Nano Systems at KTH, now at Stanford University) and KTH PhD students, and
“Now we have proven that the two crucial technologies work, independently from one another,” Ladhani says.
The results show that the technique would be capable of detecting influenza infection after the patient breathes for only a few minutes into the container. Highly-charged needles inside the bottle ionize the micro-sized droplets of water that carry the virus in the breath, and these are then attracted to an electrically-grounded droplet of liquid in the bottom of the chamber.
The next step is to break open the virus cell’s walls, using the common lab process of lysis, and then hunt down the nuclear proteins of the virus. The scientists mix the proteins in a fluid with magnetic antibody-coated beads that the proteins bind to.
The beads are flushed into the well array and when a magnet is placed underneath the array, the proteins become fluorescent, which makes them easy to detect with a camera.
“This kind of test will enable doctors to treat severely threatened patients the right way, and it will be valuable for use in clinical research,” Van Der Wijngaart says. “It’s harder than finding a needle in a haystack, but it can be done.”