Researchers at Tufts University School of Dental Medicine and the Sackler School of Graduate Biomedical Sciences at Tufts, led by Jonathan Garlick, have established for the first time that skin cells from diabetic foot ulcers can be reprogrammed to acquire properties of embryonic-like cells. These induced pluripotent stem cells might someday be used to treat chronic wounds. The study is published online in advance of print in Cellular Reprogramming.
A second study from the research team published in Wound Repair and Regeneration found that a protein called fibronectin is linked to a break-down in the wound-healing process in cells from diabetic foot ulcers.
“The results are encouraging. Unlike cells taken from healthy human skin, cells taken from wounds that don’t heal – like diabetic foot ulcers – are difficult to grow and do not restore normal tissue function,” said senior author on both studies, Jonathan Garlick, Ph.D., D.D.S., stem cell researcher at Tufts University School of Dental Medicine in Boston. “By pushing these diabetic wound cells back to this earliest, embryonic stage of development, we have “rebooted” them to a new starting point to hopefully make them into specific cell types that can heal wounds in patients suffering from non-healing wounds.”
The research team successfully reprogramed cells from diabetic wounds to an embryonic-like state and turned them into cell types that are important for wound healing. Using three independent criteria, they confirmed that the cells had been reprogrammed to a pluripotent state, which means that they can be turned into a variety of different cell types, including those that can stimulate wound repair.
As a next step, the research team created 3D engineered tissues that they had previously found to mimic many features of chronic wounds. They used these 3D tissues to test the properties of cells from diabetic foot ulcers and found that cells from diabetic ulcers get stuck making an immature scaffold made up predominantly of a protein called fibronectin that is likely to prevent proper closure of wounds. Fibronectin has been shown to be abnormal in other diabetic complications, such as kidney disease, but this is the first study that directly connects it to cells taken from diabetic foot ulcers.
“The development of more effective therapies for foot ulcers has been hampered by the lack of realistic wound-healing models that closely mimic the function of the extracellular matrix, which is the scaffold critical for wound repair in skin,” said Anna Maione, Ph.D., first author on theWound Repair and Regeneration study who did this work as part of her Ph.D. studies in Cell, Molecular & Developmental Biology at the Sackler School and her post-doctoral work at Tufts University School of Dental Medicine. “This work builds on our paper published in 2015 that showed that cells from diabetic ulcers have fundamental defects which we can simulate using our 3D tissue models grown in the lab. These models will be a great way to test new therapeutics that could improve wound healing and prevent limb amputation which can result when treatments fail.”
“The 3D model is critical because it will allow us to take these studies further. Now that we have confirmed that it’s possible to reprogram wound cells to a very early stage of development we need to study if they can turn into more mature cell types and then study them in our 3D models to see if they will improve healing of chronic wounds,” said Behzad Gerami-Naini, Ph.D., first author on the study in Cellular Reprogramming and an assistant professor at Tufts University School of Dental Medicine.
“The findings advance commonly-held assumptions about how diabetic foot ulcers develop. Most importantly, our ability to reprogram these cells gives us new treatment avenues to pursue. The big question is – since we have created induced pluripotent stem cells which we can now make into many cells types important for wound healing — will they be better for wound healing than cells originally taken from the non-healing wound?” asked Garlick, who is also a member of theCell, Molecular & Developmental Biology program faculty at the Sackler School.
More than 29 million Americans have diabetes. Diabetic foot ulcers, often resistant to treatment, are a major complication. The National Diabetes Statistics Report of 2014 stated that about 73,000 non-traumatic lower-limb amputations in 2010 were performed in adults aged 20 years or older with diagnosed diabetes, and approximately 60 percent of all non-traumatic lower-limb amputations occur in people with diabetes.
Garlick’s lab at Tufts Dental School performs research at the intersection of tissue engineering and stem cell biology. Dr. Garlick and his colleagues work to grow tissues that mimic diabetic wounds from induced pluripotent stem cells by reprogramming adult cells to grown skin that mimics a range of skin diseases, including cancer and scleroderma. Their goal is to develop experimental approaches for precision therapies that may regenerate and repair diseased or damaged tissues and organs.
Additional authors on the study in Cellular Reprograming are Avi Smith, Anna G. Maione, Olga Kashpur, and Gianpaolo Carpinito, all of Tufts University School of Dental Medicine; Aristides Veves of Beth Israel Deaconess Medical Center; and David J. Mooney of Harvard University and the Wyss Institute for Biologically Inspired Engineering.
Additional authors on the study in Wound Repair and Regeneration are Liang Liang and Olivera Stojadinovic of University of Miami Miller Medical School; Avi Smith and Elana Knight of Tufts University School of Dental Medicine; Vanessa Yanaz, a student in the Cell, Molecular and Developmental Biology program at the Sackler School; David J. Mooney of Harvard University and the Wyss Institute; Aristidis Veves of Beth Israel Deaconess Medical Center, and Marjana Tomic-Canic of the University of Miami Miller Medical School. Yanaz did this work as a Post-Baccalaureate Research Program (PREP) scholar at the Sackler School and Tufts University School of Medicine. PREP is funded by an award (GM066567) from the National Institute of General Medical Sciences of the National Institutes of Health. The program is designed to increase the number of people entering research careers from groups currently under-represented in biomedical science research.
Research reported in this news release was supported by the National Institute for Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under award number DK098055 to Dr. Garlick.
Gerami-Naini, B.; Smith, A.; Maione, A.G.; Kashpur, O.; Carpinito, G.; Veves, A.; Mooney, D.J.; and Garlick, J.A. (2016). Generation of induced pluripotent stem cells from diabetic foot ulcer fibroblasts using nonintegrative Sendai virus. Cellular Reprogramming. Accepted manuscript published online, DOI: 10.1089/cell.2015.0087.
Maione, A.G.; Smith, A.; Kashpur, O.; Yanez, V.; Knight, E.; Mooney, D.J.; Veves, A.; Tomic-Canic, M.; and Garlick, J.A. (2016). Altered ECM deposition by diabetic foot ulcer-derived fibroblasts implicates fibronectin in chronic wound repair. Wound Repair and Regeneration. Accepted manuscript published online, DOI: 10.1111/wrr.12437. Published online April 22, 2016.
About Tufts University School of Dental Medicine
Founded in 1868, Tufts University School of Dental Medicine (TUSDM) is committed to leadership in education, patient care, research, and community service. Students obtain an interdisciplinary education, integrated with medicine, with access to training in dental specialties. Clinics managed at TUSDM provide quality comprehensive care to more than 18,000 diverse individuals annually, including those with special needs. Nationally and internationally, the School promotes health and educational programs and researches new procedures, materials and technologies to improve oral health.
About Tufts University School of Medicine and the Sackler School of Graduate Biomedical Sciences
Tufts University School of Medicine and the Sackler School of Graduate Biomedical Sciences at Tufts University are international leaders in medical and population health education and advanced research. Tufts University School of Medicine emphasizes rigorous fundamentals in a dynamic learning environment to educate physicians, scientists, and public health professionals to become leaders in their fields. The School of Medicine and the Sackler School are renowned for excellence in education in general medicine, the biomedical sciences, and public health, as well as for innovative research at the cellular, molecular, and population health level. Ranked among the top in the nation, the School of Medicine is affiliated with six major teaching hospitals and more than 30 health care facilities. Tufts University School of Medicine and the Sackler School undertake research that is consistently rated among the highest in the nation for its effect on the advancement of medical and prevention science.