Research published today in Nature from scientists at Huntsman Cancer Institute (HCI) at the University of Utah shows how epithelial cells – such as skin cells – naturally turn over, maintaining constant numbers between cell division and cell death.
In addition to skin, epithelial cells comprise skin-like linings that coat internal organs, giving organs a protective barrier so they can function properly. Cells turn over very quickly in epithelia. To maintain healthy cell densities, an equal number of cells must divide and die. If that balance gets thrown off, inflammatory diseases or cancers can arise.
The study leader, Jody Rosenblatt, investigator at HCI and associate professor of oncological sciences at the University of Utah, says, “If too many epithelial cells die, you can lose the organ barrier function and inflammatory diseases like asthma and colitis can result. On the other hand, if too many cells divide compared to the number dying, this can cause an overabundance of cells, which can lead to tumor formation. So imbalance on either side is problematic.”
Around ninety percent of cancers arise in the simple epithelia that coat the organs. Understanding what normally controls cell division and death, and how these processes are linked, is essential to understanding how these events become misregulated to drive cancer formation. While scientists had previously studied cell division and death in response to experimental triggers, how these processes naturally occur was less clear.
The HCI team discovered an answer to this puzzle. They learned that opposing mechanical tensions control both cell division and cell death. Specifically, they found that stretching epithelial cells causes them to divide, and crowding epithelial cells causes them to expel and die.
“We knew there had to be some kind of regulation to tie the death and division processes together,” says Rosenblatt. “What we found boils down to really simple principles. It’s all mechanical tension. If the cells get too crowded – 1.6-fold more crowded – then they pop some cells out that later die. The extrusion of cells enables the cell sheets to return to densities they like.”
On the flip side, researchers noticed that cells divided in sparser areas. They realized those sparse regions were creating a tension on the cells to stretch.
“If the cells become too sparse, then they activate cells to divide – and that signal to divide comes from mechanical stretch,” explains Rosenblatt. “To test this, we stretched cells and found that stretch could trigger cells to divide within only one hour! The process also showed us that stretch is a normal trigger for cell division.”
Rosenblatt’s team analyzed human colon cells, zebrafish cells, and dog cell cultures. The places where the cells divided were always more stretched out – 1.6-fold more stretched out, just like the ratio for cell death.
The next question was figuring out what caused these processes to happen. Rosenblatt’s team discovered both cell division and death were controlled by the same protein, Piezo1.
“Basically this same protein is sensing both crowding and stretch – but the outcome is very different, depending on what state the cells are in,” says Rosenblatt. “Piezo1 is sort of like a thermostat, regulating two different sides. Just like a thermostat regulates both heat and cold, it makes sense to have one sensor measuring crowding and stretch. If there were two separate regulators, things could get out of hand fairly quickly if one sensor breaks.”
In addition to understanding how Piezo1 is involved in regulation, Rosenblatt’s team also identified a stage in the cell cycle where cells sit paused for repair.
“We had always assumed that once cells start a division cycle, they just power through. We didn’t know that they take breaks throughout the cell cycle,” explains Rosenblatt. “But we found a point where the cells were just stalled, waiting to divide. A lot of things need to happen for cells to divide. The DNA needs to replicate so it can divide in half, providing each new cell with the same DNA as the parent. These cells have everything ready to do that, but they still pause there at a step that we did not expect to be regulated. Cells could be paused waiting to reach a certain size. Once they reach this size, stretch triggers them to divide.”
With the insight into how cells normally divide on their own, Rosenblatt believes scientists will have better insight into how epithelial cells divide when they shouldn’t, like in cancer.
“By understanding how cell death and division are normally regulated,” she explains, “we are discovering new ways that these processes go wrong—especially in diseases we don’t currently have treatments for, things like asthma and metastatic cancers.”
This study was funded by a National Institute of Health Director’s New Innovator Award and University of Utah Funding Incentive Seed Grant, with additional support from National Cancer Institute and the Huntsman Cancer Foundation.