Kidney Fibrosis Study Reveals Causes, Potential Therapeutic Target

Research identifies key factor leading to cell cycle arrest and fibrosis production in the kidney

Kidney fibrosis

A new study of how scar tissue is produced in the kidneys has identified potential culprits and promising targets for intervention. The study, published in Science Translational Medicine, was led by HSCI scientists based at Brigham and Women’s Hospital.

Background

Chronic kidney disease is a growing global health concern, affecting about 10 percent of the world’s population. A final, common pathway in chronic kidney disease is fibrosis, the formation of internal scar tissue, which can cause devastating effects. In the kidneys, it can ultimately lead to end-stage kidney failure.

Recent research has shown that after acute kidney injury, the kidneys often fail to repair themselves completely. Kidney cells may get stuck during the point in the cell cycle where they release pro-fibrotic factors.

What the study found

HSCI faculty member Joseph Bonventre and his colleagues identified key factors involved in this cell cycle arrest and provided insight into its consequences. Based on these findings, they identified a structure within the cell that is involved in the cell cycle arrest and proposed new therapeutic targets for kidney fibrosis.

“As a disease mechanism, fibrosis may account for more deaths than any other,” said Dr. Bonventre, who is chief of the Division of Renal Medicine at Brigham and Women’s Hospital. “Our lab has been studying acute and chronic kidney injury and fibrosis for many years. We’re now focused on the transition from acute to chronic kidney disease and what leads to fibrosis in the kidneys.”

How they did it

Bonventre and colleagues studied the transition from acute to chronic kidney disease in mice, using extracted epithelial cells and analyzing which genes were active when.

They observed that as kidney fibrosis progresses, a critical compartment is formed within kidney cells: the rapamycin-autophagy spatial coupling compartment, called TASCC. (TASCCs are also formed during liver fibrosis.)

The scientists found that cyclin G1 (CG1), a protein involved in regulation of cell cycle arrest, also plays a role in triggering the formation of TASCCs.

Their experiments showed that in the presence of TASCC, the secretion of fibrosis-promoting factors increased. Blocking the formation of TASCCs reduced the severity of kidney fibrotic disease progression in preclinical models.

The studies in mice were complemented by research in a group of patients suffering from chronic kidney disease who had developed progressive fibrotic lesions.

Compared to control subjects, the kidney tissue of these patients had more kidney cells arrested in the G2/M phase of the cell cycle. These cells contained TASCCs. Further supporting the significance of this link, TASCC-containing cells were also found in patients with acute kidney injury.

Why it matters

By identifying a mechanism that triggers the formation of a compartment in kidney cells that facilitates the secretion of pro-fibrosis factors, the scientists have discovered a promising target for developing new therapies for patients with chronic kidney disease.